Spike detectability

What are the limits of spike detectability in GT conditions where we know the waveform(s) we’re looking for.

To-do

For a single channel, compute dot product between the template (average spike waveform) of that channel and each of the individual extracellular GT spikes.
For same channel, compute dot product between the template and segments of the recording.
Plot the frequency distribution of dot product results in two conditions. Can the distributions be separated?
Systematically vary threshold used in each case and plot ROC curve. Outlook: Potential for using generative models + template matching for spike detection.

Spike propagation trajectory across multiple channels

Given a probe insertion close to parallel to the somato-dendritic axis of cortex, is it possible to track spike propagation of a unit across channels?

To-do

Work out AP spatial spread along D-V axis
Map propagation trajectory for different units
Analyse features of propagation in space
Tie into sub-cellular AP signatures Outlook: Viability of investigating BAPs in vivo; use of multi-channel EAP propagation stereotipy as an added dimension for sorting.

Dark Neurons

What else can we tell about them from this dataset?

To-do

Analyse EAP and patch spike waveforms and compare to good units at similar distance range Outlook: Further characterisation of dark neurons and their identity.

Synaptic Connectivity

How can we take advantage of a paired-recording dataset to explore analyses for connectivity?

To-do

Spike sort recordings where whole-cell patch-clamp was achieved and there was a good EAP signature.
For every unit in the recording, run STA on the whole-cell unit’s membrane potential.
For every putative pre-synaptic unit, run STA and obtain extracellular footprint; investigate for AxTPs Outlook: Finding additional signatures for unit-unit connectivity.